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The Steely Group
http://www.thesteelygroup.com
http://www.thesteelygroup.com
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Scientist
Posted: 03/11/2025
2025-03-11
2025-04-17
Employment Type:
Contract
Job Category: Scientific
Job Number: 4329
Job Description
Our client is seeking a highly skilled and motivated contract Scientist to join their protein engineering team. The ideal candidate will have extensive experience in cell maintenance and expansion, transfection, and protein expression in mammalian cells.
This role requires proficiency in making buffers, protein purification chromatography (with a strong emphasis on affinity chromatography and AKTA experience), buffer exchange endotoxin measurement and analytics, aliquoting and inventory management.
Responsibilities:
This role requires proficiency in making buffers, protein purification chromatography (with a strong emphasis on affinity chromatography and AKTA experience), buffer exchange endotoxin measurement and analytics, aliquoting and inventory management.
Responsibilities:
- Cell Maintenance and Expansion: Perform routine maintenance and expansion of mammalian cell cultures to ensure optimal growth and viability.
- Transfection and Protein Expression: Transfect mammalian cell cultures for antibody/protein expression in both flasks & 96 well blocks. Track titers, troubleshoot.
- Buffer Preparation: Prepare various buffers required for protein purification and other laboratory processes.
- Protein Purification Chromatography: Execute protein purification protocols, with a focus on affinity chromatography using AKTA and Kingfisher systems.
- Buffer Exchange: Perform buffer exchange procedures, particularly using Big Tuna systems.
- Endotoxin Measurement and Cleanup: Measure and remove endotoxins from protein samples to ensure purity and safety.
- Aliquoting and Inventory Management: Accurately aliquot protein samples, manage inventory, and handle shipments to maintain an organized and efficient laboratory environment.
- Bachelor's or Master’s degree in Molecular Biology, Biochemistry, or a related field.
- Proven experience in cell culture maintenance and expansion.
- Hands-on experience with transfection and protein expression in mammalian cells.
- Proficiency in making buffers and performing protein purification chromatography, especially affinity chromatography with AKTA systems.
- Experience with buffer exchange techniques; Experience with Big Tuna instrument is a plus.
- Knowledge of endotoxin measurement and cleanup procedures.
- Strong organizational skills and attention to detail for aliquoting, inventory management, eln entry, and managing shipments.
- Experience with protein analytics (capillary electrophoresis, Octet Quantification, stability studies) are a plus
- Excellent communication and teamwork skills.
- Previous experience in a contract or temporary role within a biotechnology or pharmaceutical company is preferred.
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